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卵泡抑素样蛋白3(FSTL3)是细胞外基质糖蛋白,因为与卵泡抑素高度同源,共同拥有富含半胱氨酸的卵泡抑素位区而归属于卵泡抑素家族,参与细胞增殖、迁移、分化、胚胎发育等一系列病理生理过程。卵泡抑素样蛋白3是由FSTL3基因编码的蛋白质。它可能在白血病的发生中发挥作用。FSTL3 (Follistatin Like 3) 是一种蛋白质编码基因。 与 FSTL3 相关的疾病包括慢性白血病。 其相关途径包括 TGFB 家族成员的信号传导和胰岛素样生长因子结合蛋白 (IGFBP) 对胰岛素样生长因子 (IGF) 转运和摄取的调节。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人卵泡抑素样蛋白3(FSTL3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人卵泡抑素样蛋白3(FSTL3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human FSTL3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human FSTL3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.