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CD146 是一种属于免疫球蛋白超家族的结点粘附分子。sCD146 通过特异性与单核细胞结合,从而刺激跨内皮的迁移,参与到炎症,它也参与了血管生成。因此,sCD146 对参与血管形成的不同细胞类型表现出趋化活性,如平滑肌细胞和内皮细胞,包括祖先内皮细胞(EPC)。此外,用 sCD146 处理 EPC 导致迁移、增殖和在 Matrigel 中形成毛细血管样结构的能力增加,表明 sCD146 在内皮细胞上存在一个膜相关的结合伙伴。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人可溶性CD146分子(sCD146)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人可溶性CD146分子(sCD146)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human sCD146. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human sCD146 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.