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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
可溶性细胞程序性死亡蛋白 1 配体 2(sPD-L2)是一种蛋白质,由 PDCD1LG2 基因编码。PDCD1LG2 也被命名为 CD273(分化群 273)。PDCD1LG2 是 一种免疫检查点受体配体,在适应性免疫反应的负向调节中发挥作用。PD-L2 是程序性细胞死亡蛋白 1(PD-1)的两个已知配体之一。PD-L2 是属于 B7 蛋白家族的细胞表面受体。它由细胞外区域的免疫球蛋白样可变结构域和免 疫球蛋白样恒定结构域、一个跨膜结构域和一个细胞质结构域组成。PD-L2 与其他 B7 蛋白有相当的序列同源性,但它不包含 CD28/CTLA4 的推定结合 序列。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人可溶性细胞程序性死亡蛋白1配体2(sPD-L2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人可溶性细胞程序性死亡蛋白1配体2(sPD-L2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human sPD-L2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human sPD-L2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.