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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
含SCAN域蛋白3(SCAND3),也被称为锌指BED结构域蛋白9(ZBED9)或锌指蛋白452(ZNF452),是由ZBED9基因编码的蛋白质。它在N端包含一个扫描锌指(ZNF)结构域,在N端的侧翼包含一个INT核心和一个TP酶衍生的hATd二聚化模块。它和Myo1g基因的高甲基化是肝细胞癌的潜在诊断生物标志物。它在非小细胞肺癌 (NSCLC) 组织中高表达,表达增加与晚期疾病、淋巴结转移和不良预后相关,因此可能是候选预后标志物。 可作为癌基因并通过在G1/S期转变时对细胞周期进程的正向调节来促进肿瘤生长。它可能通过激活AKT/GSK-3 beta信号通路促进侵袭和转移。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人含SCAN域蛋白3(SCAND3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人含SCAN域蛋白3(SCAND3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SCAND3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SCAND3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.