计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
含SFT2域的蛋白1(SFT2D1)是一种多跨膜蛋白,属于SFT2家族,由195个氨基酸组成,其基因位于人类染色体6q27。该蛋白在多种物种中高度保守,包括黑猩猩、狗、牛、小鼠、大鼠、鸡、斑马鱼、果蝇和线虫。SFT2D1可能参与高尔基复合体内内吞区室来源的逆向运输囊泡的融合过程,在蛋白质运输和囊泡介导的运输中发挥作用,并可能位于细胞质、内膜系统及细胞内膜结合细胞器中。此外,SFT2D1与多种疾病相关基因位于同一染色体区域,如迟发性皮肤卟啉症、帕金森病、施蒂克勒综合征以及双相情感障碍易感性等,但其具体作用机制尚待进一步研究。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人含SFT2域的蛋白1(SFT2D1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人含SFT2域的蛋白1(SFT2D1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SFT2D1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SFT2D1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.