计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
CCAAT 增强子结合蛋白β,也被称为 CEBPb,是由 CEBPb 基因编码的蛋白 质。这种无内含子基因编码的蛋白质是一种 bZIP 转录因子,可以作为同源 二聚体与某些 DNA 调控区域结合。它还可以与相关蛋白 CEBP-alpha、 CEBP-delta 和 CEBP-gamma 形成异二聚体。编码的蛋白质在调节涉及免疫 和炎症反应的基因中很重要,并且已显示与 IL-6 基因中的 IL-1 反应元件以 及几种急性期和细胞因子基因的调节区域结合. 此外,编码的蛋白质可以结 合启动子和上游元件并刺激 I 型胶原基因的表达。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人周期素依赖性激酶6(CDK6)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人周期素依赖性激酶6(CDK6)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CDK6. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CDK6 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.