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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
垂体肿瘤转化1相互作用蛋白(PTTG1IP),也被称为PTTG1结合因子(PBF),是由PTTG1IP基因编码的蛋白质。它是一种特征不良的蛋白质。尽管它在正常组织中无处不在,但其确切功能仍然难以捉摸。它已被证明可以直接与安全蛋白和原癌基因PTTG1相互作用,从而促进其核易位和随后PTTG1对碱性成纤维细胞生长因子(bFGF)的转录激活。它可能在癌症中具有直接作用。已被证明可以调节甲状腺细胞的生长,过度表达会导致甲状腺增生和甲状腺内病变的形成。它的表达也与肿瘤复发独立相关。它也与乳腺癌有关。它的过表达和分泌会诱发细胞侵袭,这一过程对于转移性疾病的形成至关重要。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人垂体肿瘤转化1相互作用蛋白(PTTG1IP)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人垂体肿瘤转化1相互作用蛋白(PTTG1IP)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PTTG1IP. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PTTG1IP present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.