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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Lumican也被称为LUM,是一种细胞外基质蛋白,在人类中是由12号染色体上的LUM基因编码的。它是富含亮氨酸的小蛋白多糖(SLRP)家族的第二类成员,包括decorin、biglycan、fibromodulin、keratocan、epiphycan和osteoglycin。与其他SLRPs一样,lumican的分子量约为40千道尔顿,有四个主要的分子内结构域:一个由16个氨基酸残基组成的信号肽;一个含有硫酸化酪氨酸和二硫键的带负电荷的N端结构域;10个串联的富含亮氨酸的重复序列,允许lumican与其他细胞外成分如胶原蛋白结合;一个由50个氨基酸残基组成的羧基末端域。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人基膜聚糖(LUM)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人基膜聚糖(LUM)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LUM. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LUM present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.