BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
多聚体1，也被称为弹性蛋白微纤维干扰物4（EMILIN-4），是一种蛋白质，由MMRN1基因编码。多聚物是一种大量的可溶性蛋白，在血小板和血管内皮中发现。它由亚单位组成，通过链间二硫键连接，形成大小不一的同源多聚体。多聚体是一种因子V/Va结合蛋白，可能作为血小板因子V的载体蛋白，也可能具有细胞外基质或粘附蛋白的功能。最近，一种不寻常的常染色体显性出血疾病（魁北克因子V/魁北克血小板疾病）的患者被发现有血小板多聚物的缺陷。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人多聚蛋白1(MMRN1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人多聚蛋白1(MMRN1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MMRN1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MMRN1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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