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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
突触核苷酸家族包括α-突触核苷酸、β-突触核苷酸、γ-突触核苷酸和突触蛋白。这些小的、高度保守的蛋白质主要定位在大脑中,集中在突触前的神经终端。α-突触核蛋白通常是一种可溶性蛋白。在一些神经退行性疾病中,包括帕金森病(PD)、路易体痴呆症和阿尔茨海默病,异常的α-突触核蛋白形成丝状内含物(路易体和路易性神经纤维)。β-突触核蛋白和γ-突触核蛋白(synoretin)与α-突触核蛋白结合并抑制其聚集。寡聚体是所有 α 突触核蛋白类型中毒性最高的,而且可能是导致蛋白聚集和病理现象扩散的关键 。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人寡聚体α突触核蛋白(alpha-Synuclein)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人寡聚体α突触核蛋白(alpha-Synuclein)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human alpha-Synuclein. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human alpha-Synuclein present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.