计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尿苷胞苷激酶2(UCK2),也被称为尿苷单磷酸激酶2(UMPK),是由UCK2基因编码的酶。该基因编码的蛋白质催化尿苷和胞苷分别磷酸化为尿苷一磷酸和胞苷一磷酸。这是在生产RNA和DNA合成所需的嘧啶核苷三磷酸的第一步。此外,该基因的等位基因可能在介导针对B型流感嗜血杆菌的非体液免疫中起作用。它是两种人尿苷-胞苷激酶之一。另一种UCK蛋白是尿苷-胞苷激酶1(UCK1),UCK1与它共享约70%的序列同一性。虽然UCK1在包括肝脏,骨骼肌和心脏在内的各种健康组织中无处不在,但UCK2仅在胎盘组织中检测到。它主要在癌细胞和健康的胎盘组织中检测到。靶组织中的选择性表达导致它被鉴定为抗癌治疗中的靶标。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人尿苷胞苷激酶2(UCK2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人尿苷胞苷激酶2(UCK2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human UCK2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human UCK2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.