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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
序列相似家族19成员A5(FAM19A5),也被称为趋化因子样蛋白TAFA-5(TAFA-5),是由TAFA5基因编码的蛋白。该基因是TAFA家族的成员,TAFA家族由五个高度同源的基因组成,这些基因编码小的分泌蛋白质。这些蛋白质在固定位置含有保守的半胱氨酸残基,并且与CC-趋化因子家族的成员CCL3远缘相关。TAFA蛋白主要在大脑的特定区域表达,并被假定为大脑特异性趋化因子或神经因子,作为免疫和神经细胞的调节剂。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人序列相似家族19成员A5(FAM19A5)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人序列相似家族19成员A5(FAM19A5)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human FAM19A5. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human FAM19A5 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.