BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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tau蛋白（或称τ蛋白）是一组由MAPT（微管相关蛋白tau）基因的替代剪接产生的六种高可溶性蛋白的异构体。它们在其他地方不太常见，但在中枢神经系统星形胶质细胞和少突胶质细胞中有很低的表达。除微管蛋白外，细胞内还存在其他蛋白质与微管结合，这些蛋白质统称为微管相关蛋白（microtubule-associated proteins，MAPs)。一般情况下，MAPs可增加微管的稳定性，促进微管的组装，并调节微管与其他细胞成分之间的关系，是维持微管结构和功能必需的成分。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人微管关联蛋白τ(MAPT)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人微管关联蛋白τ(MAPT)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human MAPT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human MAPT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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