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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
成纤维细胞生长因子17是一种蛋白质，由FGF17基因编码。该基因所编码的蛋白是成纤维细胞生长因子（FGF）家族的成员。FGF家族成员具有广泛的有丝分裂和细胞存活活性，参与各种生物过程，包括胚胎发育细胞生长、形态发生、组织修复、肿瘤生长和侵袭。该基因被证明在小脑和大脑皮层有突出的表达。该基因的小鼠同源物被定位在前脑中线结构、中脑-后脑交界处、发育中的骨骼和发育中的动脉的特定部位，这表明其在中枢神经系统、骨骼和血管发育中的作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人成纤维细胞生长因子17(FGF17)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人成纤维细胞生长因子17(FGF17)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human FGF17. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human FGF17 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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