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Cathelicidin 抗菌肽（CAMP; CAP-18; CAP18; CRAMP; FALL-39; FALL39; HSD26; LL37）是一种多肽，主要储存在巨噬细胞和多形核白细胞（PMNs） 的溶酶体中；CAMP 基因编码多肽前体 CAP-18（18 kDa），通过蛋白酶3介导的胞外裂解加工成活性 形式 LL-37。 它是唯一在人体中发现的 Cathelicidin 家族的多肽。LL-37，具有净正电荷，是两亲性的，可以通过对带负电荷的细菌膜的静电吸引直接消除病原微生物。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人抗菌肽LL-37(LL-37)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人抗菌肽LL-37(LL-37)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LL-37. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LL-37 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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