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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
接触素 1,也被称为 CNTN1,是一种蛋白质,由 CNTN1 基因编码。该基因所编码的蛋白是免疫球蛋白超家族的成员。它是一种糖基磷脂酰肌醇(GPI) 锚定的神经元膜蛋白,作为一种细胞粘附分子发挥作用,它可能在发育中的神经系统中的轴突连接的形成中起作用。该基因有两个交替剪接的转录本变体,编码不同的异构体。与 CNTN1 相关的疾病包括肌病,先天性,康普顿- 北方和脱髓鞘多发性神经病。其相关途径包括 GPCR 信号传导和 Notch 信号传导途径(WikiPathways)。与该基因相关的基因本体论注释包括碳水化合物结合和蛋白质结合。该基因的一个重要同系物是 CNTN2。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人接触蛋白1(CNTN1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人接触蛋白1(CNTN1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CNTN1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CNTN1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.