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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
斯钙素2(STC-2),也被称为斯钙素相关蛋白,是由STC-2基因编码的蛋白质。该基因编码在多种组织中表达的分泌型同二聚体糖蛋白,并且可能具有自分泌或旁分泌功能。编码的蛋白质在其锡钙蛋白家族成员中具有保守的15个半胱氨酸残基中的10个,并仅在其丝氨酸残基上被酪蛋白激酶2磷酸化。它的C端含有一簇组氨酸残基,可能与金属离子相互作用 。该蛋白可能在调节肾脏和肠道钙和磷酸盐转运,细胞代谢或细胞钙/磷酸盐稳态中起作用。该基因的表达由雌激素诱导,并在一些乳腺癌中改变。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人斯钙素2(STC-2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人斯钙素2(STC-2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human STC-2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human STC-2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.