计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
无翅型MMTV整合位点家族成员7A,也被称为WNT7A,是由WNT7A基因编码的蛋白质。该基因是WNT基因家族的成员,该家族由编码分泌信号蛋白的结构相关基因组成。这些蛋白质与肿瘤发生和几个发育过程有关,包括胚胎发生过程中细胞命运和模式的调节。该基因参与女性生殖道前后轴的发展,并且在子宫平滑肌拍打和维持成人子宫功能方面也起着关键作用。它的突变与Fuhrmann和Al-Awadi/Raas-Rothschild/Schinzel phocomelia综合征有关。与它相关的疾病包括腓骨发育不全或发育不全,股骨弓形和多指,同音和少指趾和尺骨,缺乏,伴有严重肢体缺乏症 。其相关途径包括海奈特受体的突触前功能和Wnt信号网络。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人无翅型MMTV整合位点家族成员7A(WNT7A)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人无翅型MMTV整合位点家族成员7A(WNT7A)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human WNT7A. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human WNT7A present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.