BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
核因子κB受体激活因子(RANκ)又称TRANCE受体或TNFRSF11A，是肿瘤坏死因子受体（TNFR）分子亚家族的一员。它是RANK-配体（RANKL）的受体，是调节破骨细胞分化和激活的RANK/RANKL/OPG信号传导途径的一部分。它与骨重塑和修复、免疫细胞功能、淋巴结发育、热调节和乳腺发育有关。它的细胞质结构域与TRAFs 1、2、3、5和6结合，将信号传递给下游目标，如NF-κB和JNK。它在骨骼肌、胸腺、肝脏、结肠、小肠、肾上腺、破骨细胞、乳腺上皮细胞、前列腺、血管细胞和胰腺中表达。最常见的是，NF-κB的激活是由RANKL介导的，但仅RANK的过度表达就足以激活NF-κB途径。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人核因子κB受体激活因子(RANκ)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人核因子κB受体激活因子(RANκ)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human RANκ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human RANκ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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