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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
核纤层蛋白 B1(LMNB1)是一种蛋白质,是由 LMNB1 基因编码的。核层由 位于内核膜旁边的二维蛋白质矩阵组成。层状蛋白家族构成了该矩阵,并在 进化中高度保守。在有丝分裂过程中,随着层析蛋白的磷酸化,层析矩阵被 可逆地分解。它被认为参与了核稳定性、染色质结构和基因表达。脊椎动物 的板层蛋白包括两种类型,即 A 型和 B 型,该基因编码两种 B 型蛋白中的一 种,即 B1。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人核纤层蛋白B1(LMNB1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人核纤层蛋白B1(LMNB1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LMNB1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LMNB1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.