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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
波形蛋白(VIM)是一种结构蛋白,由 VIM 基因编码。Vimentin 是一种 III 型中间丝(IF)蛋白,在间质细胞中表达。中间丝与基于管蛋白的微管和基于肌动蛋白的微丝一起构成了细胞骨架,在所有的动物细胞和细菌中都能找到中间丝蛋白。所有的中间丝蛋白都以高度发育调节的方式表达;它是间质细胞的主要细胞骨架成分。正因为如此,在正常发育和转移过程中,波形蛋白常常被用作间质细胞或经历上皮-间质转化(EMT)的细胞的标志。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人波形蛋白(VIM)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人波形蛋白(VIM)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human VIM. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human VIM present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.