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淋巴毒素-β(LT-β)也被称为肿瘤坏死因子C(TNF-C),是一种在人类中由LTB基因编码的蛋白质。淋巴毒素β是TNF家族的一种II型膜蛋白。它通过异质三聚体的形成将淋巴毒素-α固定在细胞表面。淋巴细胞表面的主要形式是淋巴毒素-α1/β2复合物(如1分子α/2分子β),这种复合物是淋巴毒素-β受体的主要配体。次要的复合物是淋巴毒素-α2/β1。LTB是炎症反应系统的诱导剂,参与淋巴组织的正常发育。淋巴毒素-β异构体b不能与淋巴毒素-α复合,表明淋巴毒素-β的功能与淋巴毒素-α无关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人淋巴毒素β(LTb)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人淋巴毒素β(LTb)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LTb. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LTb present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.