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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
LPCAT3(溶血卵磷脂酰基转移酶 3)是一个蛋白质编码基因。其相关途径包 括 PE 的酰基链重塑和三酰甘油的生物合成。与该基因相关的基因本体论 (GO)注释包括酰基转移酶活性和1-酰基甘油磷酸胆碱O-酰基转移酶活性。该基因的一个重要类似物是 MBOAT。已发现抑制 LPCAT3 会改变细胞脂质组,并部分防止铁死亡。基于其在鸡体内同源物 (cLPCAT3) 的晶体学和冷冻电镜研究,[9] 人性 MBOAT5 与其他成员(如 SOAT1 和 DGAT1)一样具有典型的 MBOAT 折叠,[10][11] 并且跨膜螺旋具有“T”形反应室,允许溶血磷脂酰胆碱 (lysoPC) 和长多不饱和酰基辅酶 A(如花生四烯酸)共占据酰基辅酶 A。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人溶血卵磷脂酰基转移酶3(LPCAT3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人溶血卵磷脂酰基转移酶3(LPCAT3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LPCAT3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LPCAT3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.