BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
热休克蛋白 70(HSP-70)是一个保守的普遍表达的热休克蛋白家族。具有类 似结构的蛋白质几乎存在于所有生物体中。细胞内定位的 Hsp70s 是细胞的 蛋白质折叠机制的重要组成部分，执行伴侣功能，并帮助保护细胞免受生理 压力的不利影响。此外，膜结合的 Hsp70s 已被确定为癌症疗法的潜在目标， 其细胞外定位的对应物已被确定为具有膜结合和无膜结构。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人热休克蛋白70(HSP-70)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人热休克蛋白70(HSP-70)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human HSP-70. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human HSP-70 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
危险品化学品经营许可证（带存储）