计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
热休克蛋白 90(HSP-90)是一种伴侣蛋白,协助其他蛋白质正常折叠,稳 定蛋白质以对抗热应力,并协助蛋白质降解。它还能稳定肿瘤生长所需的一 些蛋白质。热休克蛋白,作为一个类别,是所有物种中表达量最高的细胞蛋 白之一。热休克蛋白在温度升高的情况下保护细胞。它们在未受压的细胞中 占总蛋白质的 1-2%。然而,当细胞被加热时,热休克蛋白的比例增加到细 胞蛋白的 4-6%。热休克蛋白 90(HSP90)是热相关蛋白中最常见的一种。” 90“即它的质量大约为 90 千道尔顿。一个 90 千道尔顿的蛋白质被认为是相 当大的非纤维蛋白。HSP90 存在于细菌和真核生物的所有分支中,但在古细 菌中显然不存在。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人热休克蛋白90(HSP-90)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人热休克蛋白90(HSP-90)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human HSP-90. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human HSP-90 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.