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阿替普酶（rt-PA）是一种重组组织型纤溶酶原激活剂，属糖蛋白类溶栓药物，通过激活纤溶酶原转化为纤溶酶，特异性降解纤维蛋白以溶解血栓，广泛用于急性缺血性卒中、心肌梗死及肺栓塞的治疗12。其分子含两个环状结构，对纤维蛋白具有高亲和力，溶栓作用强且选择性高，可减少全身性出血风险。rt-PA通过赖氨酸残基与血栓中的纤维蛋白结合，激活结合状态的纤溶酶原，促使纤溶酶生成并降解纤维蛋白，实现靶向溶栓68。该过程可能导致循环中纤维蛋白原轻度消耗，需监测凝血功能（如纤维蛋白原、APTT）以评估出血风险。临床上主要用于治疗急性心肌梗死、肺栓塞、 急性缺血性脑卒中、深静脉血栓及其他血管疾病。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人爱通立(rt-PA)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人爱通立(rt-PA)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human rt-PA. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human rt-PA present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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