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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素16(IL-16)是一种蛋白质,由IL16基因编码。该基因编码的蛋白是一种多变性细胞因子,具有化学吸引剂、T细胞激活调节剂和HIV复制抑制剂的功能。这种细胞因子的信号传导过程是由CD4介导的。该基因的产物经过蛋白质分解处理,发现产生两个功能性的蛋白质。细胞因子的功能完全归因于分泌的C端肽,而N端产物可能在细胞周期控制中起作用。据报道,Caspase 3参与了该蛋白的蛋白水解过程。据报道,有两个交替剪接的转录变体编码不同的异构体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白细胞介素16(IL-16)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素16(IL-16)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-16. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-16 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.