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白介素1δ(IL-1δ)也被称为白细胞介素36受体拮抗剂(IL-36RA),是白细胞介素36家族中的一员。该蛋白通过与其受体IL-36R/IL1RL2竞争而抑制白细胞介素-36细胞因子(IL-36α、IL-36β和IL-36γ)的作用,从而抑制其促炎作用。IL-36RN基因的突变导致有缺陷的IL-36RA蛋白的减少或产生,已被证明可导致炎症性皮肤病,包括全身性脓疱性银屑病、哈洛波持续化脓性皮炎(ACH)和急性全身性脓疱病(AGEP)。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白细胞介素36Ra(IL-36Ra;IL-1F5)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素36Ra(IL-36Ra;IL-1F5)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-36Ra;IL-1F5. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-36Ra;IL-1F5 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.