BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素27（IL-27）是由EBI3和p28亚单位通过二硫键构成的异源二聚体。IL-27可以促进Th1和Tr1分化, 抑制Th2、Th17及Treg应答, 在免疫调节方面具有重要作用。IL-27 属于IL-12家族成员,主要由抗原提呈细胞,包括树突状细胞、巨噬细胞等在微生物或其他免疫刺激物刺激下产生,其受体广泛分布于多种免疫细胞。IL-27信号通过细胞上的异源二聚体受体IL-27R传导至胞内。IL-27R由WSX-1和gp130亚基构成。WSX-1和gp130可以在包括T细胞、NK细胞、B细胞、髓系细胞等多种细胞上表达。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人白细胞介素27(IL-27)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素27(IL-27)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-27. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-27 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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