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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素2受体β是一种蛋白质,由IL2RB基因编码。也被称为CD122;IL15RB;P70-75。白介素2受体参与T细胞介导的免疫反应,在结合白介素2的能力方面有3种形式。 低亲和力形式是α亚基的单体(也叫CD25),不参与信号转导。中等亲和力的形式由γ/β亚单位的异质体组成,而高亲和力的形式由α/β/γ亚单位的异质体组成。该受体的中间和高亲和力形式都参与受体介导的内吞作用和白介素2有丝分裂信号的转导。 该基因编码的蛋白代表β亚单位,是一种I型膜蛋白。该蛋白也构成了IL-15受体的三个亚单位之一。该受体的激活会增加CD8+效应T细胞的增殖。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白细胞介素2受体β(IL-2Rβ)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素2受体β(IL-2Rβ)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-2Rβ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-2Rβ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.