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白细胞介素32(IL-32)是促炎症细胞因子,由IL32基因编码。在高等哺乳动物中可以找到,但在啮齿动物中没有。它主要在细胞内表达,蛋白质有9种不同的异构体,因为前mRNA可以交替剪接。最活跃和研究的异构体是IL-32γ。它不属于任何细胞因子家族,因为与其他细胞因子几乎没有同源性。IL-32的mRNA主要在免疫细胞中表达,但也可在其他组织中表达,如脾脏、胸腺、肺、小肠、结肠、前列腺、心脏、胎盘、肝脏、肌肉、肾脏、胰腺和大脑。IL-32是细胞因子家族的一个成员。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白细胞介素32(IL-32)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素32(IL-32)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-32. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-32 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.