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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白细胞介素9 (IL-9)是一种分子量在28-30kDa之间的单体糖基化蛋白,属于IL-7/IL-9家族,是一种多效性细胞因子,主要由CD4+T细胞产生,肥大细胞和自然杀伤细胞也能产生IL-9。最初IL-9被认为是T细胞和肥大细胞的生长因子,之后研究发现IL-9还能调节B细胞免疫球蛋白的产生、增强肥大细胞蛋白酶的表达、促进杯状细胞增生和粘液产生,表明IL-9可能参与过敏性炎症反应的发生。IL-9包含144个氨基酸,其中包括18个氨基酸的信号肽,成熟的IL-9结构中还具有10个半胱氨酸残基和4个潜在的N连接糖基化位点。IL-9与受体结合后,会激活多种STATs,调节目标基因转录。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人白细胞介素9(IL-9)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白细胞介素9(IL-9)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human IL-9. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human IL-9 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.