BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
白血病抑制因子(LIF)是一种白细胞介素6类细胞因子，通过抑制分化影响细胞生长。当LIF水平下降时，细胞会进行分化。LIF与特定的LIF受体（LIFR-α）结合，后者与该受体家族所有成员共有的特定亚基--GP130信号转导亚基形成一个异质体。这导致了JAK/STAT（Janus激酶/信号转导者和转录激活者）和MAPK（有丝分裂原激活蛋白激酶）级联的激活。LIF通常在发育中的胚胎滋养层表达，其受体LIFR在整个内细胞群中表达。由于胚胎干细胞来自囊胚阶段的内细胞团，将它们从内细胞团中移除，也就消除了其LIF的来源。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人白血病抑制因子(LIF)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人白血病抑制因子(LIF)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human LIF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human LIF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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