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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
睫状神经营养因子是一种蛋白质,由CNTF基因编码。该基因编码的蛋白是一种多肽激素和神经营养因子,其作用主要在神经系统中被研究,它能促进神经递质的合成和包括星形胶质细胞在内的某些神经群体的神经元外展。它是一种下丘脑神经肽,是神经元和少突胶质细胞的有效生存因子,可能与减少炎症发作时的组织破坏有关。该基因的突变导致异常剪接,导致睫状神经营养因子的缺乏,但这种表型与神经系统疾病没有因果关系。除了源自该基因座的主要的单克隆转录物外,该基因还与上游的ZFP91基因共转录。这两个基因座的共转录导致转录本包含锌指蛋白的完整编码区,但缺乏睫状神经营养因子的完整编码区。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人睫状神经营养因子(CNTF)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人睫状神经营养因子(CNTF)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human CNTF. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human CNTF present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.