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硫嘌呤S甲基转移酶（TPMT），也被称为硫嘌呤甲基转移酶，是由TPMT基因编码的酶，它是一种催化硫嘌呤类化合物甲基化的酶。该位点的假基因位于染色体18q上。它甲基化硫嘌呤化合物。甲基供体是S-腺苷-L-蛋氨酸，它被转化为S-腺苷-L-同型半胱氨酸。该酶通过S-腺苷-L-蛋氨酸作为S-甲基供体和S-腺苷-L-同型半胱氨酸作为副产物代谢硫嘌呤药物。该基因的缺陷导致6MP的甲基化减少和失活减少，导致骨髓毒性增强，这可能导致骨髓抑制、贫血、出血倾向、白细胞减少和感染。TPMT活性调节硫嘌呤前药的细胞毒性作用。这种酶的天然底物尚未确定。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人硫嘌呤S甲基转移酶(TPMT)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人硫嘌呤S甲基转移酶(TPMT)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TPMT. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TPMT present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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