BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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磷脂酰肌醇-4-激酶α（PI4KA），也被称为PIK4CA或磷脂酰肌醇 4-激酶 III α，是由PI4Ka基因编码的酶。该酶催化磷脂酰肌醇 4,5-二磷酸生物合成中的第一个关键步骤。哺乳动物PI 4-激酶根据其分子量分为两种类型，II型和III型，并通过洗涤剂和腺苷的调节。该基因编码的蛋白质是一种不受腺苷抑制的III型酶。已为该基因描述了编码不同同种型的两种转录变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人磷脂酰肌醇-4-激酶α(PI4Ka)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人磷脂酰肌醇-4-激酶α(PI4Ka)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PI4Ka. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PI4Ka present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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