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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α突触核蛋白(α-SYN)是一种蛋白质，由SNCA基因编码。α-突触核蛋白是一种神经元蛋白质，调节突触囊泡的运输和随后的神经递质释放。它在大脑中含量丰富，而在心脏、肌肉和其他组织中发现的数量较少。在大脑中，它主要存在于神经元顶端的特殊结构，称为突触前终端。在这些结构中，α-突触核蛋白与磷脂和蛋白质相互作用。 突触前终端从被称为突触小泡的小室中释放化学信使，称为神经递质。神经递质的释放在神经元之间传递信号，对大脑的正常功能至关重要。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人磷酸化α突触核蛋白(PSNCA)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人磷酸化α突触核蛋白(PSNCA)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PSNCA. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PSNCA present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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