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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
一氧化氮合酶1(神经元),也被称为NOS1,由NOS1基因编码。一氧化氮合酶(NOSs)是一个合酶家族,催化从L-精氨酸产生一氧化氮(NO)。一氧化氮是一种化学信使,根据其酶源和组织定位的不同,在全身具有不同的功能。在大脑和外周神经系统中,NOS1主要存在,NO显示出神经递质的许多特性,并可能参与长期的增效作用。它与中风和神经退行性疾病相关的神经毒性、平滑肌的神经调节(包括蠕动和括约肌放松)和阴茎勃起有关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人神经型一氧化氮合酶(NOS1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人神经型一氧化氮合酶(NOS1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human NOS1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human NOS1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.