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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
神经颗粒素(Neurogranin)是一种钙调蛋白结合蛋白,主要在大脑中表达,特别是在树突棘中,并参与蛋白激酶C信号传导途径。它是调节钙调蛋白可用性的主要突触后蛋白,在没有钙的情况下与之结合。蛋白激酶C的磷酸化会降低其结合能力。NRGN基因的表达受甲状腺激素的控制。人的神经粒蛋白由78个氨基酸组成。一项研究表明,神经粒蛋白基因与男性患精神分裂症的风险增加有潜在联系,另一项研究给出了精神分裂症患者大脑中神经粒蛋白免疫反应性降低的证据。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人神经颗粒素(NRGN)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人神经颗粒素(NRGN)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human NRGN. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human NRGN present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.