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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
端粒重复序列结合因子2(TERF2),也被称为TRF2和TRBF2,是由TERF2基因编码的蛋白质。它是在整个细胞周期中都存在于端粒处的蛋白质。它是庇护素核蛋白复合物的一个组成部分,也是端粒长度的第二个负调节因子,在端粒的保护活性中起关键作用。它于1997年在Titia de Lange的实验室首次报道,就Myb结构域而言,发现了与TERF1相似但不相同的DNA 序列。De Lange分离出新的含有Myb的蛋白质序列,并将其命名为TERF2。TERF2蛋白上有4个结构域类别,使其能够与Shelterin蛋白复合物中的其他蛋白质以及特定类型的DNA结合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人端粒重复序列结合因子2(TERF2)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人端粒重复序列结合因子2(TERF2)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TERF2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TERF2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.