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端锚聚合酶1(TNKS1),也被称为端锚聚合酶(TNKS),是由TNKS基因编码的酶。它抑制TERF1与端粒DNA的结合。它是一种聚ADP-核糖基转移酶,参与各种过程,如Wnt信号通路,端粒长度和囊泡运输。通过介导AXIN1和AXIN2(β-连环蛋白破坏复合物的2个关键组分)的多ADP-核糖基化(PARylation)作为Wnt信号通路的激活剂:RNF146识别多ADP-核糖基化靶蛋白,其介导它们的泛素化和随后的降解。它还介导BLZF1和CASC3的PARsylation,随后是RNF146的募集和随后的泛素化。介导TERF1的PARsylation,从而有助于端粒长度的调节。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人端锚聚合酶1(TNKS1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人端锚聚合酶1(TNKS1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TNKS1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TNKS1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.