BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
粒细胞趋化蛋白2（GCP2），也称为CXCL6，是ELR+ CXC趋化因子家族的成员。它主要由巨噬细胞、上皮细胞等产生，在炎症和免疫反应中扮演重要角色。GCP2通过结合G蛋白偶联受体CXCR1和CXCR2，激活中性粒细胞，促进中性粒细胞的趋化和血管生成。此外，GCP2还对革兰氏阴性菌和革兰氏阳性菌具有抗菌作用。在结构上，GCP2由77个氨基酸组成，其NH2-末端含有特征性的ELR基序。GCP2在多种疾病中发挥作用，如呼吸系统疾病、炎症性肠病等，是炎症过程的重要介质。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人粒细胞趋化蛋白2(GCP2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人粒细胞趋化蛋白2(GCP2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human GCP2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human GCP2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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