BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
丝氨酸/精氨酸富集剪接因子1(serine/arginine-rich splicing factor 1，SRSF1)是选择性剪接因子家族的代表性成员，参与前体mRNA的选择性剪 接与加工，胞内定位与运输等生理过程。研究已证实，SRSF1为原癌蛋白，并在肺 癌，乳腺癌和白血病等人类肿瘤细胞中存在过表达现象。SRSF1蛋白通过与肿 瘤相关基因的相互作用，调控细胞周期及凋亡等多种途径参与肿瘤的发生，发 展过程。此外，SRSF1蛋白通过影响PI3K-Akt-mTOR，Ras-MNK-MAPK及 Wnt-β-catenin信号转导通路中相关基因的剪接方式发挥致癌作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人精氨酸/丝氨酸丰富剪接因子1(SRSF1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人精氨酸/丝氨酸丰富剪接因子1(SRSF1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SRSF1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SRSF1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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