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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
糖皮质激素受体β(GR-β)是糖皮质激素受体的一类亚型,另一类是GRα。两者均为糖皮质激素受体基因同一转录产物通过不同的剪切方式剪切的结果。后者能与DNA结合,但不与类固醇结合,因此可能会通过干扰GRα与DNA结合,而成为类固醇的抑制物。GRα和GRβ的前727个氨基酸完全相同,从第728个氨基酸开始GRα有50个氨基酸序列,而GRβ只有15个氨基酸序列。在mRNA水平上,两者都包含第1到第8外显子,不同之处为GRα包含9α外显子,而GRβ包含9β外显子。GRα几乎在所有组织和细胞中均有表达,在绝大多数细胞中其含量也远远超过GRβ,且糖皮质激素主要是通过结合GRα发挥其生物学效应。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人糖皮质激素受体β(GR-β)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人糖皮质激素受体β(GR-β)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human GR-β. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human GR-β present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.