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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Ficolin-1,通常也被称为M-ficolin,是一种在人类中由FCN1基因编码的蛋白质。ficolin家族的蛋白质由一个前导肽、一个短的N端部分组成,然后是一个胶原蛋白样结构域和一个C端纤维蛋白原样结构域。ficolin的名字来自后两个结构域。类似胶原蛋白的结构域和类似纤维蛋白原的结构域也存在于其他蛋白中,如tenascins,而前者也存在于补体蛋白C1q和collectins中,后者包括甘露糖结合凝集素和肺表面活性剂蛋白。Ficolins选择性地识别乙酰化的化合物。FCN1编码的M-菲柯林主要在外周血白细胞中表达,并被推测为具有弹性蛋白结合活性的血浆蛋白。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人纤维胶凝蛋白1(FCN1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人纤维胶凝蛋白1(FCN1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human FCN1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human FCN1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.