BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
细胞外基质蛋白1是一种蛋白质，由ECM1基因编码的。该基因编码的细胞外蛋白含有半胱氨酸模式的图案，其特征是白蛋白家族的配体结合 "双环 "结构域的半胱氨酸模式。该基因映射在表皮分化复合体（EDC）之外，EDC是参与表皮分化的三个基因家族的集群。ECM1 与乳腺癌、甲状腺癌、肝细胞癌和其他癌症以及溃疡性结肠炎有关ECM-1 中的种系突变会导致遗传病类脂蛋白沉积症。对 ECM-1 的自身免疫攻击是导致硬化性苔藓的原因。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人细胞外基质蛋白1(ECM1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人细胞外基质蛋白1(ECM1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ECM1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ECM1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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