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细胞程序性死亡蛋白5(PDCD5),也被称为TF-1细胞凋亡相关蛋白19(TFAR19),是由PDCD5基因编码的蛋白质。该基因编码在细胞凋亡过程中在肿瘤细胞中表达的蛋白质,独立于细胞凋亡诱导的刺激。在细胞凋亡诱导之前,该基因产物分布在细胞核和细胞质中。一旦细胞凋亡被诱导,该蛋白质的水平就会增加,并且通过从细胞质中的重新定位,它会在细胞核中积累。虽然其确切功能尚未确定,但这种蛋白质被认为在细胞凋亡中起着早期和普遍的作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人细胞程序性死亡蛋白5(PDCD5)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人细胞程序性死亡蛋白5(PDCD5)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human PDCD5. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human PDCD5 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.