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肝细胞生长因子受体(HGFR),也称为酪氨酸蛋白激酶Met或c-Met,是一种在人类中由MET基因编码的蛋白质。该蛋白具有酪氨酸激酶活性。初级单链前体蛋白经翻译后裂解产生α和β亚基,二硫连接形成成熟受体。MET是一种单通酪氨酸激酶受体,对胚胎发育、器官生成和伤口愈合至关重要。肝细胞生长因子/散射因子(HGF/SF)及其拼接异构体(NK1,NK2)是MET受体的唯一已知配体。它通常由上皮细胞表达,而HGF/SF的表达则限于间质细胞。当HGF/SF与其同源受体MET结合时,它通过一种尚未完全理解的机制诱导其二聚化,从而导致其激活。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肝细胞生长因子受体(HGFR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肝细胞生长因子受体(HGFR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human HGFR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human HGFR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.