BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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肝配蛋白A受体3(EPHA3)是一种蛋白质，由EPHA3基因编码。该基因属于蛋白酪氨酸激酶家族的肾上腺素受体亚家族。EPH和EPH相关的受体已被认为与介导发育事件有关，特别是在神经系统。EPH亚家族的受体通常有一个单一的激酶结构域和一个包含富含Cys的结构域和2个纤维蛋白III型重复的细胞外区域。根据其胞外结构域序列的相似性和结合肾上腺素-A和肾上腺素-B配体的亲和性，肾上腺素受体被分为2组。该基因编码一种结合肾上腺素-A配体的蛋白。该基因有两个交替剪接的转录本变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人肝配蛋白A受体3(EPHA3)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肝配蛋白A受体3(EPHA3)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human EPHA3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human EPHA3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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