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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
肺表面活性物质相关蛋白 C(SP-C)是肺部表面活性剂蛋白之一。它是由 SFTPC 基因编码的。它是一种膜蛋白。它可以进一步分解为 23-aa 的螺旋状 跨膜前肽本体、成熟的分泌型 SP-C(24-58)和连接到 BRICHOS 结构域的 连接体(59-89)。肺表面活性剂 C 的前肽有一个 N 端α-螺旋段,其功能被 认为是稳定蛋白质结构,因为成熟的肽可以不可逆地从其原生的α-螺旋结构 转变为β-片状聚集体并形成淀粉样纤维。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人肺表面活性物质相关蛋白C(SP-C)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人肺表面活性物质相关蛋白C(SP-C)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human SP-C. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human SP-C present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.